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QuantaWell QuantaWell2 microplates are standard microplates coated with nanoparticles that enhance fluorescent signals via a plasmonic effect.  These specialty microplates can be used to run ELISA-like immunoassays without the need for HRP or other enzyme.   They deliver increased sensitivity and dynamic range  compared to ELISA as shown in the interleukin- 6(IL-6) example below, all with reduced assay time.   They offer:

  • increase in sensitivity compared to ELISA
  • increase in dynamic range compared to ELISA
  • rapid protocol:  complete in under 3 hours
  • can be used with most fluorophores, most plate readers, most buffers and 50% human serum

    QW2 vs ELISA

    Figure 1. Comparison of IL-6 sandwich immunoassay run on QW2 plates (red) and on ELISA (with HRP read-out in green).

Serum Compatibility.  These plates can be run in 10% fetal bovine serum (FBS) and in 50% hum serum

Recommended Dyes.  Of all of the dyes we tested, we found streptavidin-conjugated Sure-Light P1 to deliver the highest sensitivity.  Please contact us to obtain this dye.  All other dyes yield satisfactory results.

Recommended Fluorescent Plate Readers.  QuantaWell 2 plates will work well in most plate readers but will not work in the Victor (PerkinElmer) readers.

Storage.  QuantaWell 2 plates can be stored at room temperature for up to 6 months.

Additional detail.  see Application Note

 

Serum

Figure 2. Effect of 10% fetal bovine serum (FBS) and 50% human serum (HS) on IL-6 (Interleukin 6) sandwich immunoassay.

QuantaWell 3 microplates.   These microplates are coated with nanoparticles that enhance fluorescent signals via a plasmonic effect.  They contain a higher density of particles than QW2 plates and therefore deliver greater fluorescent enhancement compared to QW2 plates.  See data in Figure 3.  The coated surface of these plates is shown in this video.

Figure 3.  Comparison of QW2 and QW3 plates in IL-6 sandwich immunoassay.

Figure 3. Comparison of QW2 and QW3 plates in IL-6 sandwich immunoassay.

 

 

 

 

 

 

 

 

For pricing, availability or to arrange an evaluation of QW2 and QW3 plates, please call us at 410-779-9201 or email us at Info@plasmonixinc.com. 

 

 

 

 

 

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