QuantaWell 2 microplates are standard microplates coated with metal nanoparticles that enhance fluorescent signals via a plasmonic effect. These specialty microplates can be used to run ELISA-like immunoassays without the need for HRP or other enzyme. They deliver increased sensitivity and dynamic range compared to ELISA as shown in the interleukin- 6(IL-6) example below, all with reduced assay time. They offer:
increase in sensitivity compared to ELISA
increase in dynamic range compared to ELISA
rapid protocol: complete in under 3 hours
- can be used with most fluorophores, most plate readers, most buffers and 50% hum serum
Serum Compatibility. These plates can be run in 10% fetal bovine serum (FBS) and in 50% hum serum
Recommended Dyes. Of all of the dyes we tested, we found streptavidin-conjugated Sure-Light P1 to deliver the highest sensitivity. Please contact us to obtain this dye. All other dyes yield satisfactory results.
Recommended Fluorescent Plate Readers. QuantaWell 2 plates will work well in most plate readers but will not work in the Victor (PerkinElmer) readers.
Storage. QuantaWell 2 plates can be stored at room temperature for up to 6 months.
Additional detail. see Application Note
QuantaWell 3 microplates. These specialty microplates are similar to QuantaWell 2 plates but deliver approximately twice the fluorescence enhancement compared to QW2 plates.
For pricing, availability or for evaluation of QW2 and QW3 plates, please call us at 410-779-9201 or email us at Info@plasmonixinc.com.