QuantaWell-2 and QuantaWell-3 microplates are standard microplates coated with nanoparticles that enhance fluorescent signals via a plasmonic effect. These specialty microplates can be used to run ELISA-like immunoassays without the need for HRP or other enzyme. They deliver increased sensitivity and dynamic range compared to ELISA as shown in the interleukin- 6(IL-6) example below, all with reduced assay time. QuantaWell-3 plates deliver several-fold more sensitivity than the QuantaWell-2 plates. The coated surface of QuantaWell-3 plates is shown in this video.
Serum Compatibility. These plates can be run in 10% fetal bovine serum (FBS) and in 50% human serum
Recommended Dyes. We recommend streptavidin-conjugated Sure-Light P1 (SLP1) to deliver the highest sensitivity. However fluorescent enhancement will be obtained with any fluorescent dye with more enhancement achieved by the red dyes than the blue-green dyes.
Recommended Fluorescent Plate Readers. QuantaWell 2 plates will work well in most plate readers but will not work in the Victor (PerkinElmer) readers.
Storage. QuantaWell 2 plates can be stored at room temperature for up to 6 months.
Additional detail. see Application Note
|QuantaWell-2, 96 well||Black 96-well plate coated with plasmonic nanoparticles.||QW2-96||$ 120 per 5-pack|
|QuantaWell-2, strip-well||Black 96-well strip-well plate coated with plasmonic nanoparticles.||QW2-SW||$ 160 per 5-pack|
|QuantaWell-2, 384 well||Black 384-well plate coated with plasmonic nanoparticles||QW2-384||$ 165 per 5-pack|
|QuantaWell-3, strip-well||Black 384-well plates coated with plasmonic nanoparticles. These plates yield several-fold better fluorescence enhancement than the QW2 plates.||QW3-SW||$ 160 per 2-pack|
|Sure-Light P1 dye||Streptavidin-conjugated Sure-Light P1 dye.||SLP1||$ 60 per vial|
Figure 1. Comparison of IL-6 immunoassay run on QW2 plates (pink) and on traditional ELISA with HRP detection (green).