QuantaWell-QuantaWell2 and QuantaWell-3 microplates are standard microplates coated with nanoparticles that enhance fluorescent signals via a plasmonic effect.  These specialty microplates can be used to run ELISA-like immunoassays without the need for HRP or other enzyme.   They deliver increased sensitivity and dynamic range  compared to ELISA as shown in the interleukin- 6(IL-6) example below, all with reduced assay time.  QuantaWell-3 plates deliver several-fold more sensitivity than the QuantaWell-2 plates.  The coated surface of QuantaWell-3 plates is shown in this video.

Serum Compatibility.  These plates can be run in 10% fetal bovine serum (FBS) and in 50% human serum

Recommended Dyes.  We recommend streptavidin-conjugated Sure-Light P1 (SLP1) to deliver the highest sensitivity.  However fluorescent enhancement will be obtained with any fluorescent dye with more enhancement achieved by the red dyes than the blue-green dyes.

Recommended Fluorescent Plate Readers.  QuantaWell 2 plates will work well in most plate readers but will not work in the Victor (PerkinElmer) readers.

Storage.  QuantaWell 2 plates can be stored at room temperature for up to 6 months.

Additional detail.  see Application Note

QuantaWell-2, 96-wellBlack 96-well plate coated with plasmonic nanoparticlesQW2-96$ 160 per 5-pack



Figure 2. Effect of 10% fetal bovine serum (FBS) and 50% human serum (HS) on IL-6 (Interleukin 6) sandwich immunoassay.

Figure 1. Comparison of IL-6 immunoassay run on QW2 plates (pink) and on traditional ELISA with HRP detection (green).













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