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QuantaWell-QuantaWell2 and QuantaWell-3 microplates are standard microplates coated with nanoparticles that enhance fluorescent signals via a plasmonic effect.  These specialty microplates can be used to run ELISA-like immunoassays without the need for HRP or other enzyme.   They deliver increased sensitivity and dynamic range  compared to ELISA as shown in the interleukin- 6(IL-6) example below, all with reduced assay time.  QuantaWell-3 plates deliver several-fold more sensitivity than the QuantaWell-2 plates.  The coated surface of QuantaWell-3 plates is shown in this video.

Serum Compatibility.  These plates can be run in 10% fetal bovine serum (FBS) and in 50% human serum

Recommended Dyes.  We recommend streptavidin-conjugated Sure-Light P1 (SLP1) to deliver the highest sensitivity.  However fluorescent enhancement will be obtained with any fluorescent dye with more enhancement achieved by the red dyes than the blue-green dyes.

Recommended Fluorescent Plate Readers.  QuantaWell 2 plates will work well in most plate readers but will not work in the Victor (PerkinElmer) readers.

Storage.  QuantaWell 2 plates can be stored at room temperature for up to 6 months.

Additional detail.  see Application Note

Product
Description
Catalog #
Price
QuantaWell-2, 96 wellBlack 96-well plate coated with plasmonic nanoparticles.QW2-96$ 120 per 5-pack
QuantaWell-2, strip-wellBlack 96-well strip-well plate coated with plasmonic nanoparticles.QW2-SW$ 160 per 5-pack
QuantaWell-2, 384 wellBlack 384-well plate coated with plasmonic nanoparticlesQW2-384$ 165 per 5-pack
QuantaWell-3, strip-wellBlack 384-well plates coated with plasmonic nanoparticles. These plates yield several-fold better fluorescence enhancement than the QW2 plates. QW3-SW$ 160 per 2-pack
Sure-Light P1 dyeStreptavidin-conjugated Sure-Light P1 dye. SLP1$ 60 per vial
Serum

Figure 2. Effect of 10% fetal bovine serum (FBS) and 50% human serum (HS) on IL-6 (Interleukin 6) sandwich immunoassay.

 

Figure 3.  Comparison of QW2 and QW3 plates in IL-6 sandwich immunoassay.

Figure 3. Comparison of QW2 and QW3 plates in IL-6 sandwich immunoassay.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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