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QuantaWell QuantaWell2 microplates are standard microplates coated with metal nanoparticles that enhance fluorescent signals via a plasmonic effect.  These specialty microplates can be used to run ELISA-like immunoassays without the need for HRP or other enzyme.   They deliver increased sensitivity and dynamic range  compared to ELISA as shown in the interleukin- 6(IL-6) example below, all with reduced assay time.   They offer:

  • increase in sensitivity compared to ELISA
  • increase in dynamic range compared to ELISA
  • rapid protocol:  complete in under 3 hours
  • can be used with most fluorophores, most plate readers, most buffers and 50% hum serum

    QW2 vs ELISA

    Figure 1. Comparison of IL-6 sandwich immunoassay run on QW2 plates (red) and on ELISA (with HRP read-out in green).

Serum Compatibility.  These plates can be run in 10% fetal bovine serum (FBS) and in 50% hum serum

Recommended Dyes.  Of all of the dyes we tested, we found streptavidin-conjugated Sure-Light P1 to deliver the highest sensitivity.  Please contact us to obtain this dye.  All other dyes yield satisfactory results.

Recommended Fluorescent Plate Readers.  QuantaWell 2 plates will work well in most plate readers but will not work in the Victor (PerkinElmer) readers.

Storage.  QuantaWell 2 plates can be stored at room temperature for up to 6 months.

Additional detail.  see Application Note

 

Serum

Figure 2. Effect of 10% fetal bovine serum (FBS) and 50% hum serum (HS) on IL-6 (Interleukin 6) sandwich immunoassay.

QuantaWell 3 microplates.   These specialty microplates are similar to QuantaWell 2 plates but deliver approximately twice the fluorescence enhancement compared to QW2 plates.

For pricing, availability or for evaluation of QW2 and QW3 plates, please call us at 410-779-9201 or email us at Info@plasmonixinc.com. 

 

 

 

 

 

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