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QuantaWell QuantaWell2 microplates are standard microplates coated with metal nanoparticles that enhance fluorescent signals via a plasmonic effect.  These specialty microplates can be used to run fluorescent immunoassays and will deliver a 20 – 50-fold enhancement in the intensity of the fluorescent signal and a 2 order of magnitude increase in dynamic range (see examples below).   They offer:

  • 20x – 50x increase in fluorescent intensity
  • 2 order of magnitude increase in dynamic range
  • rapid protocol:  complete in under 3 hours
  • save money:  no need for HRP substrates
  • can be used with most fluorophores, most plate readers, most buffers and  50% human serum

Recommended Protocol.     Coat plate with 2 – 5 ug/mL of capture antibody.  Wash.  Block with 3% BSA.  Wash.  Incubate with sample or standard.  Wash.    Incubate with biotinylated detection antibody.  Wash.  Incubate with streptavidin-conjugated dye.  Wash.  Read.

Serum

Figure 1. Effect of 10% fetal bovine serum (FBS) and 50% human serum (HS) on IL-6 (Interleukin 6) sandwich immunoassay.

Serum Compatibility.  These plates can be run in 10% fetal bovine serum (FBS) and in 50% human serum (HS).  See Figure 1.

Recommended Dyes.  Of all of the dyes we tested, we found streptaviding-conjugated Sure-Light P1 to deliver the highest sensitivity.  Please contact us to obtain this dye.  All other dyes yield satisfactory results.

Recommended Fluorescent Plate Readers.  QuantaWell 2 plates will work well in most plate readers but will not work in the Victor (PerkinElmer) readers.

Storage.  QuantaWell 2 plates can be stored at room temperature for up to 6 months.

Additional detail.  see Application Note.

QuantaWell 3 microplates.   These specialty microplates are similar to QuantaWell 2 plates but deliver approximately twice the fluorescence enhancement compared to QW2 plates.

Pricing

Product
Catalog #
Availability
Pricing*
QuantaWell 2, 96-wellQW2-96from stock$ 145 per 5-pack
QuantaWell 2, strip-wellQW2-SWLimited stock$ 160 per 5-pack
QuantaWell 2, 384-wellQW2-384Upon request$ 165 per 5-pack
QuantaWell 3, 96-wellQW3-96Upon request$ 160 per 2-pack

* Please contact us for additional volume discounts

Shipping charges are additional.

Ordering Information 

Please call 410-779-9201 or fax 410-705-6452 or email (Info@plasmonixinc.com) us with your order.  QuantaWell microplates will be shipped upon receipt of your Purchase Order via overnight carrier.  Shipping charges are additional.




 For more information or to evaluate QuantaWell 2 plates, please call  Plasmonix  at 410-779-9201 or email us at info@plasmonixinc.com.

 

 

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