QuantaWell 2 microplates are standard microplates coated with metal nanoparticles that enhance fluorescent signals via a plasmonic effect. These plates can be used to run fluorescent immunoassays and will deliver a 20 – 50-fold enhancement in the intensity of the fluorescent signal and a 2 order of magnitude increase in dynamic range (see examples below). They offer:
- 20x – 50x increase in fluorescent intensity
- 2 order of magnitude increase in dynamic range
rapid protocol: complete in under 3 hours
- save money: no need for HRP substrates
- can be used with most fluorophores, most plate readers, most buffers and 50% human serum
Recommended Protocol. Coat plate with 2 – 5 ug/mL of capture antibody. Wash. Block with 3% BSA. Wash. Incubate with sample or standard. Wash. Incubate with biotinylated detection antibody. Wash. Incubate with streptavidin-conjugated dye. Wash. Read.
Serum Compatibility. These plates can be run in 10% fetal bovine serum (FBS) and in 50% human serum (HS). See Figure 1.
Recommended Dyes. Of all of the dyes we tested, we found streptaviding-conjugated Sure-Light P1 to deliver the highest sensitivity. Please contact us to obtain this dye. All other dyes yield satisfactory results.
Recommended Fluorescent Plate Readers. QuantaWell 2 plates will work well in most plate readers but will not work in the Victor (PerkinElmer) readers.
Storage. QuantaWell 2 plates can be stored at room temperature for up to 6 months.
Additional detail. see Application Note.
|QuantaWell 2, 96-well||QW2-96||from stock||$ 145 per 5-pack|
|QuantaWell 2, strip-well||QW2-SW||Limited stock||$ 160 per 5-pack|
|QuantaWell 2, 384-well||QW2-384||Upon request||$ 165 per 5-pack|
* Please contact us for additional volume discounts
Shipping charges are additional.
Please call 410-779-9201 or fax 410-705-6452 or email (Info@plasmonixinc.com) us with your order. QuantaWell microplates will be shipped upon receipt of your Purchase Order via overnight carrier. Shipping charges are additional.
For more information or to evaluate QuantaWell 2 plates, please call Plasmonix at 410-779-9201 or email us at email@example.com.